Nitrofuran(AHD) ELISA Test kit
1.Introduction:Nitrofuran ELISA test kit is based on the competitive enzyme immunoassay for the detection of Aminohydantion (AHD) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Aminohydantion (AHD) in the sample and the coupling antigens pre-coated on the micro well stripes compete for the anti AHD antibodies.Aftertheadditionoftheenzymeconjugate,the TMB substrate is added for coloration. The optical density (OD) value of the sample has an egative correlation with the AHD in it. This value is compared to the standard curve and the AHD concentration is subsequently obtained.
2. ELISA procedures procedures
1 Bring test kit to the room temperature (20 - 25 °C ) for at least 30 min, note that each reagent must be shaken evenly before use; put the required micro-well strips into plate frames. Re-sealed the unused microplate, stored at 2 - 8 °C , not frozen.
2 Solution preparation: dilute 40 mL of the concentrated washing buffer (20 × concentrated) with the distilled or deionized water to 800 mL (or just to the required volume) for use.
3 Numbering: number the micro-wells according to samples and standard solution, each testing sample and standard preparation should be performed in duplicate, record their positions.
4 Add 50 µ L of the sample or standard solution to separate duplicate wells, an d add 50 µ L of the antibody working solution into each well, seal the microplate with the cover membrane, Mix gently by shaking the plate manually, incubate at 25 °C for 1 h.
5 Pour the liquid out of micowell, flap to dry on absorbent paper, add 250 µ L/well of washing buffer to wash microplate for 10 s, repeat 4 - 5 times, then take out and flap to dry with absorbent paper .
6 Add 100 µ L of the enzyme conjugate into each well; and incubate at 25 °C for 30 min.Take out microplate, continue as described in 5.
7 Coloration: add 50 µ L of the substrate A solution and then 50 µ L of the B solution into each well.Mix gently by shaking the plate manually, and incubate at 25 °C for 30 min at dark for coloration.
8 Determination: add 50 µ L of the stop solution into each well. Mix gently by shaking the plate
manually. Set the wavelength of the microplate reader at 450 nm to determine the OD value of
every well.(Recommend to read the OD value at the dual-wavelength 450/630 nm within 5 min) .
3. Result judgment
There are two methods to judge the results: the first one is the rough judgment, while the second is the quantitative determination. Note that the OD value of the sample has a negative correlation with the AOZ in the sample.
3.1 Qualitative Qualitative Qualitative Qualitative determination determination determination determination.The concentration range (ng/mL) can be obtained from comparing the average OD value of the sample with that of the standard solution. Assuming that the OD value of the sample1 is 0.268,and that of the sample 2 is 1.230, the OD value of standard solution is: 1.671 for 0ppb, 1.425 for 0.05ppb, 1.103 for 0.15ppb, 0.567 for 0.45 ppb, 0.205 for 1.35 ppb ,0.104 for 4.05 ppb, accordingly the concentration range of the sample1 is 0.45 to 1.35, and that of the sample2 is 0.05 to 0.15ppb.
4 We have other Nitrofuran Kits
Product Code Product Name Spec Sensitivity (ppb) Sample Performance Food Safety ELISA test kit(Antibiotic drugs and toxin analysis) LSY-10001 Nitrofuran (AMOZ) ELISA Test Kit 96 wells/kit 0.1 Fish,Shrimp, Chicken/Liver, Honey LSY-10002 Nitrofuran (AOZ) ELISA Test Kit 96 wells/kit 0.02 Fish,Shrimp, Chicken/Liver, Honey LSY-10003 Nitrofuran (AHD) ELISA Test Kit 96 wells/kit 0.05 Fish,Shrimp, Chicken/Liver, Honey LSY-10004 Nitrofuran (SEM) ELISA Test Kit 96 wells/kit 0.1 Fish,Shrimp, Chicken/Liver, Honey
|FOB Price:||FOB USD 400~500 / Bag|
|Minimum Order:||1 Bag/Bags kit|
|Payment Terms:||Western Union|